KMID : 0861020070220010007
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Korea Journal of Herbology 2007 Volume.22 No. 1 p.7 ~ p.12
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Effects of Hwangryunhaedok-tang on DNA Damage, Antioxidant Enzymes Expression and Acetylcholinesterase Activity
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Moon Jin-Young
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Abstract
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Objectives : In Alzheimer¡¯s disease(AD), free radical oxidative stress caused by amyloid beta-peptide may lead to DNA damage, neuronal dysfunction, neurotoxicity and cell death, Hwangryunhaedok-tang(HHT) is traditionally used for the treatment of pyrogenetic diseases. To develop a new anti-AD drug from natural herb, HHT was selected and extracted in this study.
Methods : The antioxidant activities of HHT water extract powder were examined by hydroxyl radical-induced DNA strand nicking assay, and antioxidative enzymes expression assay in H4IIE cell. In addition, HHT was examined for the inhibitory effect on the acetylcholinesterase(AChE) using by Ellman¡¯s coupled assay.
Results: The HHT exhibit DNA protective effect in the hydroxyl radical-induced DNA Strand nicking assay, mRNA expression of superoxide dismutase and glutathione peroxidase were recovered at a normal level by HHT treatment in H4IIE cell. Furthermore, water extract of HHT showed inhibitory effect on AChE activity.
Conclusion : These results suggest that HHT may be effective in delaying and preventing AD progression related to the free radical-induced DNA damage and AChE activity.
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KEYWORD
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Hwangryunhaedok-tang, DNA damage, acetylcholinesterase, Alzheimer
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